|
| 1 | +{ |
| 2 | + "cells": [ |
| 3 | + { |
| 4 | + "cell_type": "markdown", |
| 5 | + "id": "211553de", |
| 6 | + "metadata": {}, |
| 7 | + "source": [ |
| 8 | + "# PlateAdapter\n", |
| 9 | + "\n", |
| 10 | + "`PlateAdapter` represents accessories that hold plates while providing a\n", |
| 11 | + "standardized grid of holes or wells. It is useful when a plate cannot be placed\n", |
| 12 | + "directly on a carrier site because the skirt or dimensions do not match the\n", |
| 13 | + "standard ANSI/SLAS format. Examples include magnetic racks, heater shakers or\n", |
| 14 | + "adapters for semi-skirted PCR plates.\n", |
| 15 | + "\n", |
| 16 | + "By treating adapters as resources, PyLabRobot can automatically compute the\n", |
| 17 | + "position of the plate relative to the adapter. This ensures wells line up with\n", |
| 18 | + "the adapter holes while keeping a simple API for moving plates around.\n", |
| 19 | + "\n", |
| 20 | + "In most workflows you create an adapter and then assign a plate to it." |
| 21 | + ] |
| 22 | + }, |
| 23 | + { |
| 24 | + "cell_type": "markdown", |
| 25 | + "id": "5ba42bf1", |
| 26 | + "metadata": {}, |
| 27 | + "source": [ |
| 28 | + "## Creating a PlateAdapter\n", |
| 29 | + "\n", |
| 30 | + "You can instantiate a `PlateAdapter` directly by specifying its size and the\n", |
| 31 | + "layout of its holes. The example below creates a simplified magnetic rack for a\n", |
| 32 | + "96 well plate." |
| 33 | + ] |
| 34 | + }, |
| 35 | + { |
| 36 | + "cell_type": "code", |
| 37 | + "execution_count": null, |
| 38 | + "id": "1d427527", |
| 39 | + "metadata": {}, |
| 40 | + "outputs": [], |
| 41 | + "source": [ |
| 42 | + "from pylabrobot.resources.plate_adapter import PlateAdapter\n", |
| 43 | + "\n", |
| 44 | + "magnet = PlateAdapter(\n", |
| 45 | + " name=\"magnet\",\n", |
| 46 | + " size_x=127.76,\n", |
| 47 | + " size_y=85.48,\n", |
| 48 | + " size_z=35.0,\n", |
| 49 | + " dx=9.8,\n", |
| 50 | + " dy=6.8,\n", |
| 51 | + " dz=27.5, # height of the plate above the deck\n", |
| 52 | + " adapter_hole_size_x=8.0,\n", |
| 53 | + " adapter_hole_size_y=8.0,\n", |
| 54 | + " adapter_hole_size_z=8.0,\n", |
| 55 | + " site_pedestal_z=6.2,\n", |
| 56 | + ")\n", |
| 57 | + "magnet" |
| 58 | + ] |
| 59 | + }, |
| 60 | + { |
| 61 | + "cell_type": "markdown", |
| 62 | + "id": "abbafb2c", |
| 63 | + "metadata": {}, |
| 64 | + "source": [ |
| 65 | + "## Placing a plate on an adapter\n", |
| 66 | + "\n", |
| 67 | + "`PlateAdapter.assign_child_resource()` automatically aligns the plate's wells\n", |
| 68 | + "with the adapter holes. This uses `compute_plate_location()` internally.\n", |
| 69 | + "Below we place a Corning 96 well plate on our magnetic rack." |
| 70 | + ] |
| 71 | + }, |
| 72 | + { |
| 73 | + "cell_type": "code", |
| 74 | + "execution_count": null, |
| 75 | + "id": "88b02d82", |
| 76 | + "metadata": {}, |
| 77 | + "outputs": [], |
| 78 | + "source": [ |
| 79 | + "from pylabrobot.resources.corning import Cor_96_wellplate_2mL_Vb\n", |
| 80 | + "\n", |
| 81 | + "plate = Cor_96_wellplate_2mL_Vb(name=\"plate\")\n", |
| 82 | + "magnet.assign_child_resource(plate)\n", |
| 83 | + "magnet.children" |
| 84 | + ] |
| 85 | + }, |
| 86 | + { |
| 87 | + "cell_type": "markdown", |
| 88 | + "id": "9c1f90c1", |
| 89 | + "metadata": {}, |
| 90 | + "source": [ |
| 91 | + "`compute_plate_location()` can also be called directly to see where the plate\n", |
| 92 | + "will be positioned relative to the adapter." |
| 93 | + ] |
| 94 | + }, |
| 95 | + { |
| 96 | + "cell_type": "code", |
| 97 | + "execution_count": null, |
| 98 | + "id": "3e3cbbd0", |
| 99 | + "metadata": { |
| 100 | + "lines_to_next_cell": 2 |
| 101 | + }, |
| 102 | + "outputs": [], |
| 103 | + "source": [ |
| 104 | + "magnet.compute_plate_location(plate)" |
| 105 | + ] |
| 106 | + } |
| 107 | + ], |
| 108 | + "metadata": { |
| 109 | + "jupytext": { |
| 110 | + "cell_metadata_filter": "-all", |
| 111 | + "main_language": "python", |
| 112 | + "notebook_metadata_filter": "-all" |
| 113 | + } |
| 114 | + }, |
| 115 | + "nbformat": 4, |
| 116 | + "nbformat_minor": 5 |
| 117 | +} |
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