|
1 | | -{"cells": [{"cell_type": "markdown", "id": "abb22091", "metadata": {}, "source": ["# Hello World, \"Cytomat Incubator\"!\n", "\n", "The Cytomat series of incubators is used for storing microplates under\n", "controlled environmental conditions. PyLabRobot implements the\n", "{class}`~pylabrobot.incubators.cytomat.cytomat.CytomatBackend` which\n", "supports several models such as `C6000`, `C6002`, `C2C_50`, `C2C_425`,\n", "`C2C_450_SHAKE` and `C5C`.\n", "\n", "In this tutorial we show how to:\n", "- connect to the incubator\n", "- configure racks\n", "- move plates in and out\n", "- monitor temperature and humidity\n", "\n", "```{note}\n", "This notebook uses `await` statements which must be run inside an\n", "asynchronous environment such as `asyncio`.\n", "```"]}, {"cell_type": "code", "execution_count": null, "id": "b6b9218a", "metadata": {}, "outputs": [], "source": ["from pylabrobot.incubators import CytomatBackend, CytomatType\n", "from pylabrobot.incubators.cytomat.racks import cytomat_rack_9mm_51\n", "from pylabrobot.incubators.incubator import Incubator\n", "from pylabrobot.resources.corning.plates import Cor_96_wellplate_360ul_Fb\n", "from pylabrobot.resources import Coordinate\n", "\n", "# Connect to the incubator via a serial port\n", "backend = CytomatBackend(model=CytomatType.C6000, port=\"/dev/ttyUSB0\")\n", "\n", "# Create a rack and assemble an `Incubator` resource\n", "rack = cytomat_rack_9mm_51(\"rack_A\")\n", "incubator = Incubator(\n", " backend=backend,\n", " name=\"cyto\",\n", " size_x=860,\n", " size_y=550,\n", " size_z=900,\n", " racks=[rack],\n", " loading_tray_location=Coordinate(0, 0, 0),\n", ")"]}, {"cell_type": "markdown", "id": "de3fe5c6", "metadata": {}, "source": ["## Setup\n", "\n", "Setting up the incubator opens the serial connection and initializes the\n", "device."]}, {"cell_type": "code", "execution_count": null, "id": "cee39594", "metadata": {}, "outputs": [], "source": ["await incubator.setup()"]}, {"cell_type": "markdown", "id": "8f379ea7", "metadata": {}, "source": ["## Storing a plate\n", "\n", "To store a plate we first place it on the loading tray and then call\n", "{meth}`~pylabrobot.incubators.incubator.Incubator.take_in_plate`.\n", "You can choose a site automatically or specify one explicitly.\n"]}, {"cell_type": "code", "execution_count": null, "id": "25169f81", "metadata": {}, "outputs": [], "source": ["plate = Cor_96_wellplate_360ul_Fb(\"my_plate\")\n", "incubator.loading_tray.assign_child_resource(plate)\n", "await incubator.take_in_plate(\"smallest\") # choose the smallest free site\n", "\n", "# other options:\n", "# await incubator.take_in_plate(\"random\") # random free site\n", "# await incubator.take_in_plate(rack[3]) # store at rack position 3\n"]}, {"cell_type": "markdown", "id": "31f1c241", "metadata": {}, "source": ["## Retrieving a plate\n", "\n", "Use {meth}`~pylabrobot.incubators.incubator.Incubator.fetch_plate_to_loading_tray`\n", "to move a plate from storage to the loading tray."]}, {"cell_type": "code", "execution_count": null, "id": "632ec3f7", "metadata": {}, "outputs": [], "source": ["await incubator.fetch_plate_to_loading_tray(\"my_plate\")\n", "retrieved = incubator.loading_tray.resource"]}, {"cell_type": "markdown", "id": "7dc4dfb9", "metadata": {}, "source": ["## Monitoring conditions\n", "\n", "The Cytomat provides queries for temperature and humidity."]}, {"cell_type": "code", "execution_count": null, "id": "0d768495", "metadata": {}, "outputs": [], "source": ["current_temp = await incubator.get_temperature()\n", "current_humidity = await incubator.backend.get_humidity()\n", "print(current_temp, current_humidity)"]}, {"cell_type": "markdown", "id": "7c27b01e", "metadata": {}, "source": ["## Shutdown\n", "\n", "Always close the connection when finished."]}, {"cell_type": "code", "execution_count": null, "id": "28b68d42", "metadata": {}, "outputs": [], "source": ["await incubator.stop()"]}], "metadata": {"jupytext": {"cell_metadata_filter": "-all", "main_language": "python", "notebook_metadata_filter": "-all"}}, "nbformat": 4, "nbformat_minor": 5} |
| 1 | +{ |
| 2 | + "cells": [ |
| 3 | + { |
| 4 | + "cell_type": "markdown", |
| 5 | + "id": "abb22091", |
| 6 | + "metadata": {}, |
| 7 | + "source": [ |
| 8 | + "# Hello World, \"Cytomat Incubator\"!\n", |
| 9 | + "\n", |
| 10 | + "The Cytomat series of incubators is used for storing microplates under\n", |
| 11 | + "controlled environmental conditions. PyLabRobot implements the\n", |
| 12 | + "{class}`~pylabrobot.incubators.cytomat.cytomat.CytomatBackend` which\n", |
| 13 | + "supports several models such as `C6000`, `C6002`, `C2C_50`, `C2C_425`,\n", |
| 14 | + "`C2C_450_SHAKE` and `C5C`.\n", |
| 15 | + "\n", |
| 16 | + "In this tutorial we show how to:\n", |
| 17 | + "- connect to the incubator\n", |
| 18 | + "- configure racks\n", |
| 19 | + "- move plates in and out\n", |
| 20 | + "- monitor temperature and humidity\n", |
| 21 | + "\n", |
| 22 | + "```{note}\n", |
| 23 | + "This notebook uses `await` statements which must be run inside an\n", |
| 24 | + "asynchronous environment such as `asyncio`.\n", |
| 25 | + "```" |
| 26 | + ] |
| 27 | + }, |
| 28 | + { |
| 29 | + "cell_type": "code", |
| 30 | + "execution_count": null, |
| 31 | + "id": "b6b9218a", |
| 32 | + "metadata": {}, |
| 33 | + "outputs": [], |
| 34 | + "source": [ |
| 35 | + "from pylabrobot.incubators import CytomatBackend, CytomatType\n", |
| 36 | + "from pylabrobot.incubators.cytomat.racks import cytomat_rack_9mm_51\n", |
| 37 | + "from pylabrobot.incubators.incubator import Incubator\n", |
| 38 | + "from pylabrobot.resources.corning.plates import Cor_96_wellplate_360ul_Fb\n", |
| 39 | + "from pylabrobot.resources import Coordinate\n", |
| 40 | + "\n", |
| 41 | + "# Connect to the incubator via a serial port\n", |
| 42 | + "backend = CytomatBackend(model=CytomatType.C6000, port=\"/dev/ttyUSB0\")\n", |
| 43 | + "\n", |
| 44 | + "# Create a rack and assemble an `Incubator` resource\n", |
| 45 | + "rack = cytomat_rack_9mm_51(\"rack_A\")\n", |
| 46 | + "incubator = Incubator(\n", |
| 47 | + " backend=backend,\n", |
| 48 | + " name=\"cyto\",\n", |
| 49 | + " size_x=860,\n", |
| 50 | + " size_y=550,\n", |
| 51 | + " size_z=900,\n", |
| 52 | + " racks=[rack],\n", |
| 53 | + " loading_tray_location=Coordinate(0, 0, 0),\n", |
| 54 | + ")" |
| 55 | + ] |
| 56 | + }, |
| 57 | + { |
| 58 | + "cell_type": "markdown", |
| 59 | + "id": "de3fe5c6", |
| 60 | + "metadata": {}, |
| 61 | + "source": [ |
| 62 | + "## Setup\n", |
| 63 | + "\n", |
| 64 | + "Setting up the incubator opens the serial connection and initializes the\n", |
| 65 | + "device." |
| 66 | + ] |
| 67 | + }, |
| 68 | + { |
| 69 | + "cell_type": "code", |
| 70 | + "execution_count": null, |
| 71 | + "id": "cee39594", |
| 72 | + "metadata": {}, |
| 73 | + "outputs": [], |
| 74 | + "source": [ |
| 75 | + "await incubator.setup()" |
| 76 | + ] |
| 77 | + }, |
| 78 | + { |
| 79 | + "cell_type": "markdown", |
| 80 | + "id": "8f379ea7", |
| 81 | + "metadata": {}, |
| 82 | + "source": [ |
| 83 | + "## Storing a plate\n", |
| 84 | + "\n", |
| 85 | + "To store a plate we first place it on the loading tray and then call\n", |
| 86 | + "{meth}`~pylabrobot.incubators.incubator.Incubator.take_in_plate`.\n", |
| 87 | + "You can choose a site automatically or specify one explicitly.\n" |
| 88 | + ] |
| 89 | + }, |
| 90 | + { |
| 91 | + "cell_type": "code", |
| 92 | + "execution_count": null, |
| 93 | + "id": "25169f81", |
| 94 | + "metadata": {}, |
| 95 | + "outputs": [], |
| 96 | + "source": [ |
| 97 | + "plate = Cor_96_wellplate_360ul_Fb(\"my_plate\")\n", |
| 98 | + "incubator.loading_tray.assign_child_resource(plate)\n", |
| 99 | + "await incubator.take_in_plate(\"smallest\") # choose the smallest free site\n", |
| 100 | + "\n", |
| 101 | + "# other options:\n", |
| 102 | + "# await incubator.take_in_plate(\"random\") # random free site\n", |
| 103 | + "# await incubator.take_in_plate(rack[3]) # store at rack position 3\n" |
| 104 | + ] |
| 105 | + }, |
| 106 | + { |
| 107 | + "cell_type": "markdown", |
| 108 | + "id": "31f1c241", |
| 109 | + "metadata": {}, |
| 110 | + "source": [ |
| 111 | + "## Retrieving a plate\n", |
| 112 | + "\n", |
| 113 | + "Use {meth}`~pylabrobot.incubators.incubator.Incubator.fetch_plate_to_loading_tray`\n", |
| 114 | + "to move a plate from storage to the loading tray." |
| 115 | + ] |
| 116 | + }, |
| 117 | + { |
| 118 | + "cell_type": "code", |
| 119 | + "execution_count": null, |
| 120 | + "id": "632ec3f7", |
| 121 | + "metadata": {}, |
| 122 | + "outputs": [], |
| 123 | + "source": [ |
| 124 | + "await incubator.fetch_plate_to_loading_tray(\"my_plate\")\n", |
| 125 | + "retrieved = incubator.loading_tray.resource" |
| 126 | + ] |
| 127 | + }, |
| 128 | + { |
| 129 | + "cell_type": "markdown", |
| 130 | + "id": "7dc4dfb9", |
| 131 | + "metadata": {}, |
| 132 | + "source": [ |
| 133 | + "## Monitoring conditions\n", |
| 134 | + "\n", |
| 135 | + "The Cytomat provides queries for temperature and humidity." |
| 136 | + ] |
| 137 | + }, |
| 138 | + { |
| 139 | + "cell_type": "code", |
| 140 | + "execution_count": null, |
| 141 | + "id": "0d768495", |
| 142 | + "metadata": {}, |
| 143 | + "outputs": [], |
| 144 | + "source": [ |
| 145 | + "current_temp = await incubator.get_temperature()\n", |
| 146 | + "current_humidity = await incubator.backend.get_humidity()\n", |
| 147 | + "print(current_temp, current_humidity)" |
| 148 | + ] |
| 149 | + }, |
| 150 | + { |
| 151 | + "cell_type": "markdown", |
| 152 | + "id": "7c27b01e", |
| 153 | + "metadata": {}, |
| 154 | + "source": [ |
| 155 | + "## Shutdown\n", |
| 156 | + "\n", |
| 157 | + "Always close the connection when finished." |
| 158 | + ] |
| 159 | + }, |
| 160 | + { |
| 161 | + "cell_type": "code", |
| 162 | + "execution_count": null, |
| 163 | + "id": "28b68d42", |
| 164 | + "metadata": {}, |
| 165 | + "outputs": [], |
| 166 | + "source": [ |
| 167 | + "await incubator.stop()" |
| 168 | + ] |
| 169 | + } |
| 170 | + ], |
| 171 | + "metadata": { |
| 172 | + "jupytext": { |
| 173 | + "cell_metadata_filter": "-all", |
| 174 | + "main_language": "python", |
| 175 | + "notebook_metadata_filter": "-all" |
| 176 | + }, |
| 177 | + "language_info": { |
| 178 | + "name": "python" |
| 179 | + } |
| 180 | + }, |
| 181 | + "nbformat": 4, |
| 182 | + "nbformat_minor": 5 |
| 183 | +} |
0 commit comments