diff --git a/CHANGELOG.md b/CHANGELOG.md
index e206925782..175c44737b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -42,6 +42,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   ([#3882](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3882))
 - `opentelemetry-instrumentation-aiohttp-server`: delay initialization of tracer, meter and excluded urls to instrumentation for testability
   ([#3836](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3836))
+- Replace Python 3.14-deprecated `asyncio.iscoroutinefunction` with `inspect.iscoroutinefunction`. 
+  ([#3880](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3880))
 
 ## Version 1.38.0/0.59b0 (2025-10-16)
 
diff --git a/instrumentation/opentelemetry-instrumentation-aiokafka/src/opentelemetry/instrumentation/aiokafka/__init__.py b/instrumentation/opentelemetry-instrumentation-aiokafka/src/opentelemetry/instrumentation/aiokafka/__init__.py
index 32837b23c5..d4662c9329 100644
--- a/instrumentation/opentelemetry-instrumentation-aiokafka/src/opentelemetry/instrumentation/aiokafka/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-aiokafka/src/opentelemetry/instrumentation/aiokafka/__init__.py
@@ -95,7 +95,7 @@ async def produce():
 
 from __future__ import annotations
 
-from asyncio import iscoroutinefunction
+from inspect import iscoroutinefunction
 from typing import TYPE_CHECKING, Collection
 
 import aiokafka
diff --git a/instrumentation/opentelemetry-instrumentation-aiokafka/tests/test_instrumentation.py b/instrumentation/opentelemetry-instrumentation-aiokafka/tests/test_instrumentation.py
index c005920368..892689b853 100644
--- a/instrumentation/opentelemetry-instrumentation-aiokafka/tests/test_instrumentation.py
+++ b/instrumentation/opentelemetry-instrumentation-aiokafka/tests/test_instrumentation.py
@@ -261,11 +261,21 @@ async def async_consume_hook(span, *_) -> None:
     async def test_getone_consume_hook(self) -> None:
         async_consume_hook_mock = mock.AsyncMock()
 
+        def is_async_consume_hook_mock(obj: Any) -> bool:
+            return obj is async_consume_hook_mock
+
         AIOKafkaInstrumentor().uninstrument()
-        AIOKafkaInstrumentor().instrument(
-            tracer_provider=self.tracer_provider,
-            async_consume_hook=async_consume_hook_mock,
-        )
+
+        # mock.patch is a hack for Python 3.9 see https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3880
+        with mock.patch(
+            "opentelemetry.instrumentation.aiokafka.iscoroutinefunction"
+        ) as iscoro:
+            iscoro.side_effect = is_async_consume_hook_mock
+
+            AIOKafkaInstrumentor().instrument(
+                tracer_provider=self.tracer_provider,
+                async_consume_hook=async_consume_hook_mock,
+            )
 
         consumer = await self.consumer_factory()
         self.addAsyncCleanup(consumer.stop)
@@ -448,11 +458,20 @@ async def test_send_baggage(self) -> None:
     async def test_send_produce_hook(self) -> None:
         async_produce_hook_mock = mock.AsyncMock()
 
+        def is_async_produce_hook_mock(obj: Any) -> bool:
+            return obj is async_produce_hook_mock
+
         AIOKafkaInstrumentor().uninstrument()
-        AIOKafkaInstrumentor().instrument(
-            tracer_provider=self.tracer_provider,
-            async_produce_hook=async_produce_hook_mock,
-        )
+        # mock.patch is a hack for Python 3.9 see https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3880
+        with mock.patch(
+            "opentelemetry.instrumentation.aiokafka.iscoroutinefunction"
+        ) as iscoro:
+            iscoro.side_effect = is_async_produce_hook_mock
+
+            AIOKafkaInstrumentor().instrument(
+                tracer_provider=self.tracer_provider,
+                async_produce_hook=async_produce_hook_mock,
+            )
 
         producer = await self.producer_factory()
         self.addAsyncCleanup(producer.stop)
diff --git a/instrumentation/opentelemetry-instrumentation-aiopg/src/opentelemetry/instrumentation/aiopg/aiopg_integration.py b/instrumentation/opentelemetry-instrumentation-aiopg/src/opentelemetry/instrumentation/aiopg/aiopg_integration.py
index 4e6257fbb1..948050818c 100644
--- a/instrumentation/opentelemetry-instrumentation-aiopg/src/opentelemetry/instrumentation/aiopg/aiopg_integration.py
+++ b/instrumentation/opentelemetry-instrumentation-aiopg/src/opentelemetry/instrumentation/aiopg/aiopg_integration.py
@@ -1,4 +1,4 @@
-import asyncio
+import inspect
 import typing
 from collections.abc import Coroutine
 
@@ -197,7 +197,7 @@ async def __aenter__(self):
 
     async def __aexit__(self, exc_type, exc, t_b):
         try:
-            if asyncio.iscoroutinefunction(self._obj.close):
+            if inspect.iscoroutinefunction(self._obj.close):
                 await self._obj.close()
             else:
                 self._obj.close()
diff --git a/instrumentation/opentelemetry-instrumentation-httpx/src/opentelemetry/instrumentation/httpx/__init__.py b/instrumentation/opentelemetry-instrumentation-httpx/src/opentelemetry/instrumentation/httpx/__init__.py
index 2a82a123f9..67dc9aa960 100644
--- a/instrumentation/opentelemetry-instrumentation-httpx/src/opentelemetry/instrumentation/httpx/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-httpx/src/opentelemetry/instrumentation/httpx/__init__.py
@@ -225,8 +225,8 @@ async def async_response_hook(span, request, response):
 
 import logging
 import typing
-from asyncio import iscoroutinefunction
 from functools import partial
+from inspect import iscoroutinefunction
 from timeit import default_timer
 from types import TracebackType
 
diff --git a/instrumentation/opentelemetry-instrumentation-httpx/tests/test_httpx_integration.py b/instrumentation/opentelemetry-instrumentation-httpx/tests/test_httpx_integration.py
index e6506d290a..909ec33009 100644
--- a/instrumentation/opentelemetry-instrumentation-httpx/tests/test_httpx_integration.py
+++ b/instrumentation/opentelemetry-instrumentation-httpx/tests/test_httpx_integration.py
@@ -16,6 +16,7 @@
 
 import abc
 import asyncio
+import inspect
 import typing
 from unittest import mock
 
@@ -1086,7 +1087,7 @@ def test_custom_tracer_provider(self):
         def test_response_hook(self):
             response_hook_key = (
                 "async_response_hook"
-                if asyncio.iscoroutinefunction(self.response_hook)
+                if inspect.iscoroutinefunction(self.response_hook)
                 else "response_hook"
             )
             response_hook_kwargs = {response_hook_key: self.response_hook}
@@ -1133,7 +1134,7 @@ def test_response_hook_sync_async_kwargs(self):
         def test_request_hook(self):
             request_hook_key = (
                 "async_request_hook"
-                if asyncio.iscoroutinefunction(self.request_hook)
+                if inspect.iscoroutinefunction(self.request_hook)
                 else "request_hook"
             )
             request_hook_kwargs = {request_hook_key: self.request_hook}