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Optimize qualimap performance with multi-threaded name sorting
Pre-sort BAM files by read name using samtools with multi-threading before running qualimap rnaseq. This reduces qualimap execution time by >50% (from ~85 min to <40 min) by offloading the single-threaded sorting to the multi-threaded samtools sort with the -n flag, then passing the sorted BAM to qualimap with the --sorted flag. Resolves #1356 🤖 Generated with [Claude Code](https://claude.com/claude-code) Co-Authored-By: Claude <noreply@anthropic.com>
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modules/nf-core/qualimap/rnaseq/nextflow.config

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workflows/rnaseq/main.nf

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@@ -47,6 +47,7 @@ include { MULTIQC } from '../../modules/nf-core/multiqc'
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include { BEDTOOLS_GENOMECOV as BEDTOOLS_GENOMECOV_FW } from '../../modules/nf-core/bedtools/genomecov'
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include { BEDTOOLS_GENOMECOV as BEDTOOLS_GENOMECOV_REV } from '../../modules/nf-core/bedtools/genomecov'
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include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index'
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include { SAMTOOLS_SORT as SAMTOOLS_SORT_QUALIMAP } from '../../modules/nf-core/samtools/sort'
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//
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// SUBWORKFLOW: Consisting entirely of nf-core/modules
@@ -539,8 +540,15 @@ workflow RNASEQ {
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//
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if (!params.skip_qc) {
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if (!params.skip_qualimap) {
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QUALIMAP_RNASEQ (
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// Sort BAM by name for qualimap (performance optimization)
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SAMTOOLS_SORT_QUALIMAP (
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ch_genome_bam,
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ch_fasta.map { [ [:], it ] }
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)
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ch_versions = ch_versions.mix(SAMTOOLS_SORT_QUALIMAP.out.versions.first())
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QUALIMAP_RNASEQ (
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SAMTOOLS_SORT_QUALIMAP.out.bam,
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ch_gtf.map { [ [:], it ] }
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)
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ch_multiqc_files = ch_multiqc_files.mix(QUALIMAP_RNASEQ.out.results.collect{it[1]})

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